Comprehensive Analysis and the Characterization of Multidrug Resistant (MDR) and Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria Isolates in Clinical Samples from Some Hospitals in Asaba Delta State

Abstract

This study looked at the bacterial isolates that produced extended spectrum β-lactamase (ESBL) and were multidrug resistant (MDR) in clinical samples from the Federal Medical Center in Asaba. Between November 2018 and January 2019, 32 pure cultures of isolates from wound swabs, urine, feces, high vaginal swabs (HVS), and ear swabs were collected from the hospitals (with patient agreement). The isolates were tested for ESBL production using the double-disc synergy test and were presumed to be identified using normal culture and biochemical procedures. 16S rRNA partial sequence analysis was used to molecularly validate the identities of the chosen isolates. The disk diffusion technique, as outlined by the Clinical and Laboratory Standard Institute, was used to assess and interpret the antibiogram of a subset of isolates.